BIOL 380
Tom Huff, Instructor
thuff@gmu.edu

This experiment will examine the concentrations of iron in several "samples." We will be using an iron lamp on the flame atomic absorption spectrophotometer. The instructor will demonstrate the software and the procedures for preparing the instrument.

Each group will run a series of calibration standards to generate a calibration curves. The concentrations are given below. Then, one of the "unknown" samples (A, B or C) should be run. The printer will generate a report of the concentration of calcium in the sample. Be sure to note which sample you use! Once your group has a concentration for the unknown, use the same calibration curve to test hot tap water a nd then cold tap water.

Your laboratory report should describe the functions of the AAS and describe the procedures for preparing the instrument for use. The report should include a printout graph of the calibration curve that you have made. Indicate the data points for the c alibration standards and your sample.

Last update: February 18, 1998.