Class II A/B3 Biological Safety Cabinets

Introduction

The biological safety Cabinet is designed to provide three basic types of protection:

• Personnel protection from harmful agents inside the cabinet.
• Product protection to avoid contamination of the work, experiment or process.
• Environmental protection from contaminants contained within the cabinet.

Biological safety cabinets are divided into three classifications:

Classification	Biosafety Level	Application
Class I	1,2,3	low to moderate risk biological agents
Class II	1,2,3	low to moderate risk biological agents
Class III	4	high risk biological agents

Class I - Personnel and Environmental Protection Only
Class II - Product, Personnel and Environmental Protection

The Class II biological safety cabinet has three key features:

• A front access opening with carefully maintained inward airflow.
• HEPA-filtered, vertical, unidirectional airflow within the work area.
• HEPA-filtered exhaust air to the room or exhaust to a facility exhaust system.

The Class II, Type A/B3 cabintes are now classified as Class II, Type A2.
General Description:

• 70% air recirculated; 30% exhausted from a common plenum to the room;

• 100FPM intake;
• Biologically contaminated plenum under negative pressure or surrounded by negative pressure

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