Packard BioChip Technologies ScanArray Express microarray acquisition system is a scanning laser confocal fluorescence microscope that is used to determine the fluorescence intensity of a two- dimensional array of spots on a bio-chip or microarray. Operation of ScanArray Express involves the measurement of the fluorescence intensity of each spot on a sample. Sample spots are typically 50-200 um in diameter and a few um thick on top of the substrate surface. The microarry is scanned (with a user settable resolution) to generate an image which indicates the fluorescence intensity for each point or pixel on the array. Typically, two (or more) different fluorophores are used to determine the concentration of the items of interest in each sample. This requires that the substrate is scanned two (or more) times with different lasers to provide different excitation wavelengths. Each scan is performed on a sequential basis and the data stored. ScanArray Express 1.0 allows for simple quantitation of up to two scanned images. After scans are completed a composite image is automatically formed. An array pattern is then established so that the spots can be quantitated. The ScanArray Express system consists of two majoy software subsystems, ScanArray Express client software and ScanArray Express instrument server software. ScanArray Express instrument server software. LocationThe ScanArray is located at the Prince William Campus in SRIF Lab 221of Discovery Hall Building.
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